Philsuma wrote:got a PM on this and I'm hoping that it's ok to answer this in a thread, so that everyone can help out or learn.
"okay so the article states:Make new CXs (cultures) once a week when the newest flies emerge. (Use these flies). Then it states, "New cultures should be made from more than one boom from separate cultures. Try to take flies from at least three different booming cultures, and make separate NEW cultures from these - thus maximizing your chances of success with the new culture and production."
Do I use the newest flies or takes flies from three different booming cultures? What flies do you use to seed your cultures ?
I took a airtight sterilite bin, filled it almost full with water, and stick a fish tank heater in it. That way, I can place my cultures on top of the heated bin, thus giving me a warm culture. I put the heater at 75 degrees temp. So my culture is nice and warm.
Than, I place fresh mite paper on top of the lid, before I place the culture. After the culture is nicely place, not touching each other of course, I get another sterilite bin, remove the lid, and place that over all the cultures. Its like a dome covering the cultures. That way, the cultures is always nice and moist, due to the humidity.
I just can never get the culture to last more than the initial hatching.
If you can help that would be great. I am using josh media with new cups and vented lids everytime.
1. I make new cultures every single week. I pick a day of the week - Sunday is my day for instance, and I never, ever, ever skip or miss this day. Even if you have only 2 frogs, I would still make a new culture every single week, otherwise, at some point, you WILL have a gap or a crash.
2. There are different ideas about using flies from 2nd emergences ect. I tend to use flies from the 2nd emergence. The other benefit of having multiple cultures is to ALWAYS make cultures from DIFFERENT existing cultures. This way, you are playing the odds that by using 3 different existing or "master" cultures....you have much better odds that at least 1 or 2 of the new cultures will be excellent in production.
3. 75F is a very good temp, if kept steady.
4. I do not recommend keeping cultures in water. Having them in or so close to water, will change the dynamic of FF production and water and increased humidity is often worse than too dry.
5. Vented lids are a must and you are good to go with that. Are they from Josh's ? Do not stack the cultures on top of each other - that counteracts / inhibits the ventilation.
Try for some good pics of everything to post here. Pics allow us to assist you much better...and quicker.